In the match, RCB's Virat and De Villiers, both scored centuries and shared a 229-run partnership to help their side win the game. The money raised will be split in half between charities working in South Africa and India.
"I'm glad to be doing this with my very dear friend @abdevilliers17," Kohli shared on Instagram on Monday.
In a heartfelt post, De Villiers shared the details of the auction.
"Cricket has given me many incredible memories - and, among the most precious, stands the partnership with @virat.kohli , playing for RCB and against Gujarat Lions in 2016," AB de Villiers wrote.
"Everything clicked on what was a truly unforgettable night at the IPL. The capacity crowd at the M. Chinnaswamy stadium were going crazy, and we both scored centuries in a partnership of 229 runs from 96 balls. More importantly, RCB won the match by 144 runs," he added.
"Now we find ourselves in a global crisis caused by the Covid-19 pandemic, and Virat and I would like to help people in need, people struggling to put food on the table," de Villiers wrote.
"So we are donating some of the key items from that special day in 2016," he wrote.